Antibody Microarrays for Protein Expression Levels Detection

In the ever-evolving field of proteomics, scientists and researchers continually seek innovative techniques to unravel the complexities of protein expression within biological systems. One such cutting-edge technology is antibody microarrays, which have revolutionized the landscape of protein expression level detection. Antibody microarrays represent a powerful tool in the arsenal of proteomics research. These microarrays are designed to capture and quantify the expression levels of numerous proteins simultaneously, offering a high-throughput and multiplexed approach to protein analysis.

Design and Fabrication of Antibody Microarrays

The first step involves the careful selection of antibodies, ensuring their specificity and affinity for the target proteins. These antibodies are then immobilized onto the solid substrate in a spatially organized manner, creating an array of capture agents. The precision and accuracy of this immobilization process are paramount to the performance of the microarray.

Process of Antibody Microarrays for Protein Expression Levels Detection

• Antibody Immobilization. A diverse panel of antibodies, each specific to a particular protein of interest, is immobilized onto a solid substrate.
• Sample Incubation. The biological sample is applied onto the array, allowing proteins within the sample to bind to their respective immobilized antibodies.
• Washing and Detection. Unbound proteins are washed away, leaving only the proteins of interest bound to the array. Then employed to quantify the protein levels using some detection methods, such as fluorescence or chemiluminescence.

Features of Antibody Microarrays for Protein Expression Levels Detection

• Customizability. We can customize antibody microarrays by providing antibodies against proteins targeted by our customers according to their different research needs.
• High throughput. Our products can analyze a large number of proteins at the same time, which greatly speeds up experiments and data collection.
• Small volume sample needs. Our antibody microarrays require only a small volume of sample, which greatly helps customers save valuable sample materials.
• Versatility. Traditional methods often require separate assays for each protein, consuming precious time and resources. In contrast, antibody microarrays enable the simultaneous measurement of hundreds to thousands of proteins, providing a comprehensive view of the cellular proteome. We can study multiple proteins in a single experiment, helping our customers to greatly improve their research efficiency.

Application of Antibody Microarrays for Protein Expression Levels Detection

• Biomarker Discovery. Antibody microarrays have played a pivotal role in biomarker discovery. By comparing protein expression profiles between healthy and diseased individuals, potential biomarkers for diseases such as cancer, Alzheimer's, and diabetes can be identified. These biomarkers hold promise for early diagnosis and monitoring of disease progression.
• Drug Development. In drug development, antibody microarrays are instrumental in assessing the effects of candidate drugs on protein expression. This aids in understanding drug mechanisms, identifying off-target effects, and optimizing drug formulations.
• Systems Biology. Researchers utilize antibody microarrays can unravel the complexities of cellular signaling pathways and regulatory networks. Through study changes in protein expression under different conditions, insights into disease mechanisms and cellular responses are gained.

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