Stable Isotope Labeled Full Length MS Protein Standard

Stable isotope-labeled (SIL) amino acids standards combined with liquid chromatography-mass spectrometry (LC-MS) enable highly accurate quantification of target proteins and antibodies, which is essential for areas like biotech, life science, proteomics, and biopharmaceuticals. SIL amino acid standards have been widely used and are usually added to the sample after protein separation and digestion.  Although this approach has been validated for LC-MS/MS variability, variation in trypsin digestion has not been addressed. To minimize the influence of such variability, conditions of trypsin digestion were established to ensure maximum protein digestion, but suffer certain limitations. In such cases, the use of full-length protein as SIL internal standards would be considered superior to SIL amino acids.

Our SIL Full-Length Internal Standards

With our excellent R&D teams, advanced instruments, and rich production experiences, Creative Proteomics provides stable isotope-labeled full-length protein internal standards with the highest purity in the industry. Our innovative product portfolio can meet the needs of different protein quantification methods. If you can’t find any product, please send a request to us for a custom synthesis of the peptide/protein.

These proteins are usually supplied with the following specs

Chemical Purity> 80% as determined by SDS-PAGE and Coomassie blue staining
Expression HostHuman HEK293 cells
Amino Acid Labeled[U- 13C6, 15N4]-L-Arg and [U- 13C6, 15N2]-L-Lys
Quality controlLiquid chromatography and mass spectrometry
* This product is For Research Use Only. Do Not use in diagnostic or therapeutic procedures.

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